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  • Article number: CT032
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    Monoclonal antibody to rat IFN-gamma (clone DB-1)

    Description The mouse anti-rat IFN-γ monoclonal antibody (clone DB-1) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and neutralizes both natural and recombinant rat IFN-γ and mouse IFN-γ in vitro and in vivo. In Western blot analysis the DB-1 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity. Clone DB-1 has shown not to bind with rat IFN-α and IFN-β nor human IFNs. Further, biotinyated DB-1 antibody (cat. no. CT035) is highly effective as detector antibody in both sandwich ELISA and ELISPOT assays using DB-12 (cat no. CT052) as coating antibody. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) or biotinylated polyclonal antibody to rat IFN-γ as detector antibody can be used in an ELISA and ELISPOT systems. The DB-1 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular stain
    Host Mouse
    Application ELISA,Enzyme-linked immunosorbent spot (ELISPOT),Immunohistochemistry on frozen sections (IHC-Fs),Neutralization (Neut),Western Blot (WB)
    Unit 0 mg
    more info
    Normal leadtime 8 days
    Calculated total €325,00 
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